Epithalon Dosing & Protocols — Research Reference

Epithalon (Epitalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) modelled on the pineal peptide epithalamin. It is studied in telomerase-activation, melatonin-rhythm, and longevity research models. Unlike most peptides, Epithalon dosing is reported almost exclusively in short, pulsed cycles.

Reconstitution for Research

Epithalon is supplied lyophilized, commonly in 10 mg or 20 mg vials. A 10 mg vial reconstituted with 2 mL bacteriostatic water yields 5 mg/mL; 10 IU on a U-100 syringe at that concentration delivers 500 mcg. Refrigerate at 2–8 °C and use within the COA's documented window.

Reference Dose Ranges in Published Research

| Research model tier | Typical range | Notes | |---|---|---| | Entry / characterization | 5 mg per day | Threshold dose in most cellular and translational reports | | Standard | 5–10 mg per day | Most cited tier across telomerase-activation work | | Pulse research | 10 mg per day for short cycles | Used in melatonin-rhythm and pineal-model studies |

Scheduling

The defining feature of Epithalon protocols is the short, pulsed cycle: published research typically reports 10-day or 20-day administration windows separated by months of washout. This pattern reflects the proposed mechanism — long-lasting epigenetic and gene-expression changes that persist beyond plasma clearance.

• Most-cited cycle structures:
• 10-day cycle: 5–10 mg/day subcutaneous, repeated quarterly or biannually.
• 20-day cycle: 5 mg/day subcutaneous, repeated 1–2× per year.

Both subcutaneous and intramuscular routes appear in the published record; SC is more common.

Quality and Identity Verification

Mass-spec (LC-MS) confirmation of molecular weight (390.35 Da) and HPLC purity ≥98% are the standard acceptance criteria. The short, achiral-prone sequence (Ala-Glu-Asp-Gly) is straightforward to verify but easy to misidentify against the closely related precursor epithalamin — confirm tetrapeptide identity, not the longer parent.

Research Use Only. All content is for laboratory research and educational reference. Compounds discussed are not intended for human or veterinary consumption, prophylactic, or therapeutic use.

References

1. Khavinson VK, Bondarev IE, Butyugov AA. Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells. Bull Exp Biol Med. 2003;135(6):590–592.
2. Khavinson VK, Morozov VG. Peptides of pineal gland and thymus prolong human life. Neuro Endocrinol Lett. 2003;24(3-4):233–240.
3. Anisimov VN, Khavinson VK. Peptide bioregulation of aging: results and prospects. Biogerontology. 2010;11(2):139–149.